Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids : a review (2024)

Gray, M. J., Chang, D., Zhang, Y., Liu, J., & Bensoussan, A. (2010). Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids : a review. Biomedical Chromatography, 24(1), 91-103. https://doi.org/10.1002/bmc.1287

Gray, Michael J. ; Chang, Dennis ; Zhang, Ying et al. / Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids : a review. In: Biomedical Chromatography. 2010 ; Vol. 24, No. 1. pp. 91-103.

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title = "Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids : a review",

abstract = "The development of liquid chromatography-mass spectrometry (LC-MS) and tandem MS/MS for the analysis of bioactive components and their metabolites of herbal medicines in biological fluids is reviewed with the aim of providing an overview of the current techniques and methods used. The issues and challenges associated with various stages of the analytical method development are discussed using Ginkgo biloba and Panax ginseng as case studies. LC-MS offers selectivity and specificity in both the chromatographic separation and detection steps. This is necessary in order to measure compounds at extremely low concentrations as is often observed in plasma and urine samples. Traditional methods of detection (UV-visible) do not offer sufficient selectivity and specificity needed. The strategies and pitfalls involved with the measurement of such compounds are discussed in this review. Matrix effects, 'unseen' matrix suppression and enhancement ionization effects can significantly reduce the accuracy and precision of the measurement. The impact of the correct choice of chromatography column formats on signal-to-noise ratio is also discussed. Analytical methods from sample preparation to mass spectrometric detection is outlined in order to provide good direction for analysts intent on the measurement of bioavailable compounds from herbal medicines in plasma and urine samples.",

author = "Gray, {Michael J.} and Dennis Chang and Ying Zhang and Jianxun Liu and Alan Bensoussan",

year = "2010",

doi = "10.1002/bmc.1287",

language = "English",

volume = "24",

pages = "91--103",

journal = "Biomedical Chromatography",

issn = "0269-3879",

publisher = "John Wiley & Sons",

number = "1",

}

Gray, MJ, Chang, D, Zhang, Y, Liu, J & Bensoussan, A 2010, 'Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids : a review', Biomedical Chromatography, vol. 24, no. 1, pp. 91-103. https://doi.org/10.1002/bmc.1287

Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids : a review. / Gray, Michael J.; Chang, Dennis; Zhang, Ying et al.
In: Biomedical Chromatography, Vol. 24, No. 1, 2010, p. 91-103.

Research output: Contribution to journal › Article › peer-review

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AU - Gray, Michael J.

AU - Chang, Dennis

AU - Zhang, Ying

AU - Liu, Jianxun

AU - Bensoussan, Alan

PY - 2010

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N2 - The development of liquid chromatography-mass spectrometry (LC-MS) and tandem MS/MS for the analysis of bioactive components and their metabolites of herbal medicines in biological fluids is reviewed with the aim of providing an overview of the current techniques and methods used. The issues and challenges associated with various stages of the analytical method development are discussed using Ginkgo biloba and Panax ginseng as case studies. LC-MS offers selectivity and specificity in both the chromatographic separation and detection steps. This is necessary in order to measure compounds at extremely low concentrations as is often observed in plasma and urine samples. Traditional methods of detection (UV-visible) do not offer sufficient selectivity and specificity needed. The strategies and pitfalls involved with the measurement of such compounds are discussed in this review. Matrix effects, 'unseen' matrix suppression and enhancement ionization effects can significantly reduce the accuracy and precision of the measurement. The impact of the correct choice of chromatography column formats on signal-to-noise ratio is also discussed. Analytical methods from sample preparation to mass spectrometric detection is outlined in order to provide good direction for analysts intent on the measurement of bioavailable compounds from herbal medicines in plasma and urine samples.

AB - The development of liquid chromatography-mass spectrometry (LC-MS) and tandem MS/MS for the analysis of bioactive components and their metabolites of herbal medicines in biological fluids is reviewed with the aim of providing an overview of the current techniques and methods used. The issues and challenges associated with various stages of the analytical method development are discussed using Ginkgo biloba and Panax ginseng as case studies. LC-MS offers selectivity and specificity in both the chromatographic separation and detection steps. This is necessary in order to measure compounds at extremely low concentrations as is often observed in plasma and urine samples. Traditional methods of detection (UV-visible) do not offer sufficient selectivity and specificity needed. The strategies and pitfalls involved with the measurement of such compounds are discussed in this review. Matrix effects, 'unseen' matrix suppression and enhancement ionization effects can significantly reduce the accuracy and precision of the measurement. The impact of the correct choice of chromatography column formats on signal-to-noise ratio is also discussed. Analytical methods from sample preparation to mass spectrometric detection is outlined in order to provide good direction for analysts intent on the measurement of bioavailable compounds from herbal medicines in plasma and urine samples.

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JO - Biomedical Chromatography

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Gray MJ, Chang D, Zhang Y, Liu J, Bensoussan A. Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids : a review. Biomedical Chromatography. 2010;24(1):91-103. doi: 10.1002/bmc.1287